Vitamin D

A simple blood test that checks your vitamin D stores to support bones, muscles, and overall wellbeing.

Last reviewedJune 16, 2026
Serum
sample type
~5 mL
blood needed
~7 days
results in app
Any time of day
best timing
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Privately insured? German PKV usually reimburses.
In short

Vitamin D (measured as 25-hydroxyvitamin D) is the storage form of vitamin D and the best marker of your overall vitamin D status. Your skin makes it from sunlight, and you also get it from food and supplements.

It helps your gut absorb calcium and phosphate, which keeps bones and muscles strong, and it plays a role in immune function.

Vitamins & Minerals
Reviewed against DGKL reference practice.
Why it matters

Why test this?

Low vitamin D is very common in Germany and central Europe, especially in winter. It can weaken bones, contribute to muscle aches, and over time raises the risk of osteoporosis and fractures.

Very high levels are almost always caused by taking too much supplement and can push calcium too high. Testing tells you where you sit and whether supplementing makes sense.

Reference ranges

What is a normal result?

Aniva reads your result against research-backed ranges, not just the lab's wide normal. The reference shown below is specific to this biomarker.

Status25-OH vitamin D (SI)
Deficientbelow 50 nmol/L
Insufficient50 to 75 nmol/L
Sufficient75 to 125 nmol/L

Ranges are guidance only and thresholds differ between expert bodies. 50 nmol/L (20 ng/mL) is a widely used deficiency cut off. Read your result against your own lab's interval. To convert, 1 ng/mL equals 2.5 nmol/L.

Ranges are guidance and vary by lab and assay, aligned with DGKL practice. Always read your result against your own lab's reference interval.
What you'll learn

What insights will this test give you?

  • Whether your vitamin D is deficient, insufficient, or sufficient
  • A clear basis for deciding on supplementation and dose
  • Context for bone health, muscle aches, or low mood in winter
  • A baseline to recheck after a few months of supplements
What affects your level

What can affect this result?

What can skew the result

Levels swing with season and sun exposure and are usually lowest in late winter. Recent high dose supplements raise the number. Some assays measure D2 and D3 differently, which can shift results between labs. No fasting needed.

Best interpreted with

Read alongside calcium, phosphate, and parathyroid hormone (PTH) when bone health or calcium balance is the question.

How testing works

How is this tested?

Sample
Serum
Blood needed
~5 mL
Method
Immunoassay
Best timing
Any time of day
FAQ

Common questions

What does my vitamin D result mean? It reflects your body’s vitamin D stores. Low levels suggest you may need more intake, sun, or a supplement plan.

Do I need to fast for this test? No. You can eat and drink normally unless your clinician gives other instructions.

What can affect my result? Recent supplements, sun exposure, season, skin tone, age, pregnancy, and some medicines can change levels. High-dose biotin may skew some tests.

How often should I test? If starting or changing supplements, or at higher risk, consider retesting after a few months or as advised by your clinician.

How long do results take? Results are usually ready in about 7 days.

What should I discuss with my clinician? Your symptoms, diet, sun habits, and medications, plus whether to adjust supplements and recheck calcium or PTH.

On this page
Why testReference rangesWhat you'll learnWhat affects itHow testing worksSourcesFAQ
✦ Privately insured? German PKV usually reimburses.

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